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TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

  • Cat.No.:E-CK-A331

To Purchase E-CK-A331

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Detection principle

When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

General Information

Application DNA Fragmentation
Detection method HRP-DAB
Sample type Paraffin section, frozen section, cell slide
Assay time 3 hours
Detection instrument Light microscope
Other reagents required 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene,Ethanol,DNase I,PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120),Neutral gum(E-IR-R118)
Storage -20°C, shading light. Refer to the Manual.
Shipping Ice bag
Expiration date 12 months

Citations

  1. Frontiers in Bioengineering and Biotechnology (2022) IF: 6.064
    Scutellarin Protects Against Mitochondrial Reactive Oxygen Species-Dependent NLRP3 Inflammasome Activation to Attenuate Intervertebral Disc Degeneration.

    DOI: 10.3389/fbioe.2022.883118

  2. PHARMAZIE (2021) IF: 1.267
    The effects of the renin inhibitor aliskiren on neuronal toxic damage

    DOI: 10.1691/ph.2021.1729

  3. Medicina-Lithuania (2021) IF: 2.43
    May Argyrophilic Nucleolar Organizer Regions Be Used as a Biomarker for the Detection of the Degree of Ischemic Damage Instead of Tunel in Testicular Torsion?

    DOI: 10.3390/medicina57111177

  4. JOURNAL OF MOLECULAR HISTOLOGY (2021) IF: 2.611
    Prednisolone induces osteocytes apoptosis by promoting Notum expression and inhibiting PI3K/AKT/GSK3β/β-catenin pathway

    DOI: 10.1007/s10735-021-10006-0

    PMID: 34297260

  5. BIOLOGICAL TRACE ELEMENT RESEARCH (2020) IF: 2.639
    In Vitro and In Vivo Pretreatment with Selenium Mitigates Tetrahydrocannabinol-Induced Testicular Cell Apoptosis: the Role of AKT and p53 Pathways

    DOI: 10.1007/s12011-020-02322-5

    PMID: 32815089

    Sample: Cell Sample
  6. JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2021) IF: 2.945
    Silencing circRNA LRP6 down‐regulates PRMT1 to improve the streptozocin‐induced pancreatic β-cell injury and insulin secretion by sponging miR-9-5p

    DOI: 10.1007/s10863-021-09895-3

    Sample: Cell Sample
  7. CELL BIOLOGY INTERNATIONAL (2022) IF: 3.612
    Rifaximin protects SH-SY5Y neuronal cells from iron overload-induced cytotoxicity via inhibiting STAT3/NF-κB signaling

    DOI: 10.1002/cbin.11776

    PMID: 35143099

  8. JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849
    The protective effect of zinc on morphine-induced testicular toxicity via p53 and Akt pathways: An in vitro and in vivo approach

    DOI: 10.1016/j.jtemb.2021.126776

    PMID: 33984544

    Sample: Cell Culture Supernatant
  9. INTERNATIONAL IMMUNOPHARMACOLOGY (2021) IF: 4.932
    Influence of hyperocclusion on the remodeling of gingival tissues

    DOI: 10.1016/j.intimp.2021.107885

  10. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
    Oxidized high-density lipoprotein enhances endocrine disorders and ovarian damage in rats

    DOI: 10.1111/jcmm.16197

    PMID: 34346538

  11. Journal of Inflammation Research (2021) IF: 6.922
    Endothelin-1/Endothelin Receptor Type A-Angiopoietins/Tie-2 Pathway in Regulating the Cross Talk Between Glomerular Endothelial Cells and Podocytes in Trichloroethylene-Induced Renal Immune Injury

    DOI: 10.2147/JIR.S301104

    Sample: Serum,Plasma,Urine
  12. Cells (2022) IF: 7.666
    Regulation Networks of Non-Coding RNA-Associated ceRNAs in Cisplatin-Induced Acute Kidney Injury

    DOI: 10.3390/cells11192971

    PMID: 36230932

  13. Journal of Translational Medicine (2022) IF: 8.44
    Effects of hydrogen-rich saline in neuroinflammation and mitochondrial dysfunction in rat model of sepsis-associated encephalopathy

    DOI: 10.1186/s12967-022-03746-4

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